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At Antibody Analytics we have made a strategic decision to focus our expertise on addressing some of the scientific challenges faced during the global COVID-19 pandemic.

As experts in bioassay development, we are establishing a variety of SARS-CoV-2 related methods to support the development of therapeutics to combat this virus.

Neutralization assays with S Protein-pseudotyped lentiviral particles

 

Antibody Analytics have developed a screening assay to characterise the ability of therapeutic antibodies or small molecule inhibitors to block the entry of SARS-CoV-2 into healthy cells. We have established an in vitro assay using pseudotyped virus expressing the spike (S) protein of SARS-CoV-2 and human cells expressing ACE2 – the viral entry receptor for SARS-CoV-2. This assay is suitable for the assessment of:

  • Therapeutic antibodies which aim to inhibit viral entry
  • Small molecule inhibitors, targeted towards ACE2, the S protein or the priming proteases essential for S protein-mediated viral entry
  • Assessment of sera for neutralising antibody responses (e.g. from experimental vaccines)

In this in vitro model of the infection process, the pseudotyped virus expresses a luciferase reporter gene, facilitating medium throughput screening of compound/antibody libraries and sera.

This assay provides a complementary, orthogonal approach to our Cell Fusion Screening format.

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Cell fusion screening assay

 

Exploiting an observed coronavirus-induced phenomenon, Antibody Analytics is developing a cell fusion assay to assess the ability of molecules or antibodies to interfere with the interaction of the viral spike (S) protein from SARS-CoV-2 and ACE2 (the human receptor essential for viral entry).  Cells expressing ACE2 are co-incubated with cells expressing the viral S protein. Cell-cell fusion occurs, generating a fluorescent signal. Blocking this interaction provides a measurable reduction in fluorescence.

This assay is suitable for the assessment of:

    • Therapeutic antibodies which aim to inhibit viral entry
    • Small molecule inhibitors, targeted towards ACE2, the S protein or the priming proteases essential for S protein-mediated viral entry
    • Assessment of sera for neutralising antibody responses (e.g. from experimental vaccines)

This assay provides a complementary, orthogonal approach to our pseudotyped S protein screening assay format.

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COVID-19 Testing and Screening Solutions